Molecular Cytogenetics Core Facility
The Molecular Cytogenetics Core Facility (MCCF) at AKU-CRM provides state-of-the-art chromosome analysis services for research. It performs testing on a wide range of samples such as peripheral or cord blood, bone marrow and cell lines.
The facility is equipped with a Zeiss Metasystems automated imaging platform that is coupled with a high-resolution CCD camera and dedicated software tools. Ikaros Software allows karyotyping whereas Isis allows FISH analysis and Multicolor Karyotyping. Both the Ikaros and Isis are connected to a Metafer slide scanning platform that allows an unrivalled degree of imaging automation and quality.
Metaphase chromosome preparation - cell culture and harvesting: Culture of mammalian cells, harvest and fixation of samples for further evaluation
Ploidy evaluation: Counting of chromosomes in metaphase spreads of any species using on DAPI staining and full karyotyping based inverted DAPI
Conventional Karyotyping: G-banding that allows all chromosomes to be analysed in a single cell
Interphase and metaphase DNA fluorescence in situ hybridization (FISH): (commercial probes only)
- Multicolour FISH analysis:
Services in this facility are provided by trained and dedicated staff on a fee for service basis. For more information and assistance, please contact:
Assistant Manager, Laboratory