The core performs routine histology and specialized histochemical staining and also assists in setting up experimental design for researchers. This core facility also performs other functions including:
This core performs immunostaining on single or tissue microarray (TMA) tissue sections using an Automated Autostainer. The core staff also assists in setting up experimental design for the researchers. The functions of this facility include:
Immunostaining with established antibodies
Optimization of new antibodies
Multiple chromogens such as DAB, Fast Red
High-Throughput Automated IHC staining on DAKO Autostainer
The immunohistochemistry core is equipped with a state-of-the-art DAKO Autostainer Link 48. This is a compact, bench-top open system for staining immunohistochemical slides, controlled by protocols in the DAKO Link software. It delivers flexibility and efficiency required in research as well as clinical environment thereby improving workflow process.
Tissue MicroArray (TMA) core facility
The TMA core facility provides automated technology that allows evaluation of many tissues on a single slide while minimizing issues of variability, reproducibility and experimental bias. It also facilitates rapid analysis of multiple markers on an array of samples that are embedded on a single slide. This technology provides efficient utilization of resources while saving time.
The TMA core facility is equipped with an Automated Tissue Microarray Quick Ray Master (UATM-272A). This instrument can pick up the sample tissue from the donor blocks and insert into the correspondent recipient block.
TMA can be used for in-situ hybridization of RNA and DNA, immunofluorescence, Fluorescent in situ hybridization (FISH) and immunohistochemistry. The recipient blocks are available in three different core sizes of 1mm, 2mm, and 3mm.
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