Pathology Core Facilities 


The CRM have advanced, fully operational and well-equipped pathology core facilities that cater services in support of basic, translational and clinical research at AKU.  

The CRM pathology core is providing services in three main areas. 


The core performs routine histology and specialized histochemical staining and also assists in setting up experimental design for researchers. This core facility also performs other functions including: 

  • Tissue mamosphere and prostasphere fixation, processing, and embedding 

  • Paraffin and frozen sectioning 

  • Sectioning for RT-PCR or other molecular studies 

  •   Decalcification of bone and cartilage 
  • Special histological stains such as Trichrome, Alcian blue, Vonkossa, Oil Red O, Giemsa, Goldner’s stain 


This core performs immunostaining on single or tissue microarray (TMA) tissue sections using an Automated Autostainer. The core staff also assists in setting up experimental design for the researchers. The functions of this facility include: 

  • Immunostaining with established antibodies 

  • Optimization of new antibodies 

  • Multiple chromogens such as DAB, Fast Red 

  • High-Throughput Automated IHC staining on DAKO Autostainer 

The immunohistochemistry core is equipped with a state-of-the-art DAKO Autostainer Link 48. This is a compact, bench-top open system for staining immunohistochemical slides, controlled by protocols in the DAKO Link software. It delivers flexibility and efficiency required in research as well as clinical environment thereby improving workflow process. 

Tissue MicroArray (TMA) core facility 

The TMA core facility provides automated technology that allows evaluation of many tissues on a single slide while minimizing issues of variability, reproducibility and experimental bias. It also facilitates rapid analysis of multiple markers on an array of samples that are embedded on a single slide. This technology provides efficient utilization of resources while saving time. 

The TMA core facility is equipped with an Automated Tissue Microarray Quick Ray Master (UATM-272A). This instrument can pick up the sample tissue from the donor blocks and insert into the correspondent recipient block. 

T​MA can be used for in-situ hybridization of RNA and DNA, immunofluorescence, Fluorescent in situ hybridization (FISH) and immunohistochemistry. The recipient blocks are available in three different core sizes of 1mm, 2mm, and 3mm. 

Back to Core Facilities

For queries, please contact Dr Azhar Hussain, Director Labs and Core Facilities, CRM at