Pathology Core Facilities
We have advanced, fully operational and well-equipped pathology core facilities to support basic, translational and clinical research at AKU-CRM.
The pathology core is providing services in three main areas:
- Tissue MicroArray (TMA)
This core performs routine histology and specialised histochemical staining and also assists in setting up experimental design for researchers. This core facility also performs other functions including:
- Tissue, mammosphere and prostasphere fixation, processing, and embedding
- Paraffin and frozen sectioning
- Sectioning for RT-PCR or other molecular studies
- Decalcification of bone and cartilage
- Special histological stains such as Trichrome, Alcian blue, Vonkossa, Oil Red O, Giemsa, Goldner’s stain, etc.
This core performs immunostaining on single or tissue microarray (TMA) tissue sections using an automated autostainer. The functions of this facility include:
- Immunostaining with established antibodies
- Optimisation of new antibodies
- Multiple chromogens such as DAB, Fast Red
- High-throughput automated IHC staining on DAKO autostainer
The immunohistochemistry core is equipped with a state-of-the-art DAKO Autostainer Link 48. This is a compact, bench-top open system for staining immunohistochemical slides, controlled by protocols in the DAKO Link software. It delivers flexibility and efficiency required in research as well as clinical environment thereby improving workflow process.
We have trained core staff available to assist researchers with setting up experimental design.
Tissue MicroArray (TMA) core facility
The TMA core facility provides automated technology that allows evaluation of many tissues on a single slide while minimising issues of variability, reproducibility and experimental bias. It also facilitates rapid analysis of multiple markers on an array of samples that are embedded on a single slide. This technology enables the efficient utilisation of resources while saving time.
TMA can be used for in-situ hybridisation of RNA and DNA, immunofluorescence, Fluorescent in situ hybridisation (FISH) and immunohistochemistry.
Services in this facility are provided on a fee for service basis. For more information and assistance, please contact:
Assistant Manager, Laboratory