Fluorescence-Activated Cell Sorting (FACS) of living cells separates a population of cells into subpopulations based on fluorescent labelling. Cells stained using fluorophore-conjugated antibodies can be separated based on fluorescent recognition using specialised lasers.
FACS can be used to carry out many functions including:
- High-speed cell sorting
- Apoptotic assays
- Cell cycle analysis
The FACS Core Facility at AKU-CRM is equipped with the most advanced and state of the art cell sorters that include:
- BD FACSAria III SORP (Special Order Research Product), and
- BD FACSMelody
BD FACSAria III
BD FACSAria III can perform multi-parameter flow cytometric analysis with high throughput cell sorting. The equipment currently is capable of doing 9 colours and up to 4-way sorting. It is accompanied by BD FACSDiva software for analysis.
BD FACSMelody can perform multi-parameter flow cytometric analysis and cell sorting. The equipment currently is capable of doing 6 colours and up to 4-way sorting. It is accompanied by BD FACSChorus and BD FACSDiva software for data acquisition and analysis respectively.
Services in this facility are provided by trained and dedicated staff on a fee for service basis. For more information and assistance, please contact:
Assistant Manager, Laboratory