Confocal Microscopy Core
Confocal microscopy applies optical imaging techniques probed with various fluorescence thereby increasing optical resolution and contrast of a micrograph through a spatial pinhole to eliminate out-of-focus light.
It enables the reconstruction of three-dimensional structures from sets of images obtained at different depths within a thick object. It is widely used in numerous biological disciplines, from cell biology and genetics to microbiology and developmental biology.
The confocal microscope can be used to undertake:
- 3D imaging
- Visualization of protein-protein interactions and translocation
- Fluorescent Recovery After Photobleaching (FRAP)
- Fluorescence Loss in Photobleaching (FLIP)
- Fluorescence Localization after Photobleaching (FLAP)
Nikon Eclipse Ti-E Inverted Microscope
CRM is equipped with a Nikon Eclipse T1 inverted microscope and a CO2 chamber that can be used for live imaging and time-lapse experiments.
Back to Imaging Core Facilities
Back to Core Facilities
For queries, please contact Dr Azhar Hussain, Director Labs and Core Facilities, CRM at