Confocal Microscopy Core

Confocal microscopy applies optical imaging techniques probed with various fluorescence thereby increasing optical resolution and contrast of a micrograph through a spatial pinhole to eliminate out-of-focus light. 

It enables the reconstruction of three-dimensional structures from sets of images obtained at different depths within a thick object. It is widely used in numerous biological disciplines, from cell biology and genetics to microbiology and developmental biology. 

The confocal microscope can be used to undertake: ​​

  • 3D imaging 
  • Visualization of protein-protein interactions and translocation 
  • Fluorescent Recovery After Photobleaching (FRAP) 
  • Fluorescence Loss in Photobleaching (FLIP) 
  • Fluorescence Localization after Photobleaching (FLAP) 

 

Nikon Eclipse Ti-E Inverted Microscope 

CRM is equipped with a Nikon Eclipse T1 inverted microscope and a CO2 chamber that can be used for live imaging and time-lapse experiments. 


 

Back to Imaging Core Facilities ​

Back to Core Facilities​


 

For queries, please contact Dr Azhar Hussain, Director Labs and Core Facilities, CRM at azhar.hussain@aku.edu