Clinical Procedures

SCRAPING (MOUTH, PHARYNX, BUCCAL, TONGUE AND VISUALISED LESIONS)

Principle:
To provide diagnosis for malignant (Inflammatory lesion)

Procedure:

1. If the lesion is moist.
1. Open the bottle of fixative.
2. Scrape the lesion with the tongue depressor.
3. Smear the material onto a glass slide and immediately fix in 95% alcohol / spray fix.
4. Stain with H&E / Pap stain.
2. If the lesion is dry or has a necrotic and inflammatory surface.
   1. Open the bottle of fixative.
   2. Gently moisten and remove the necrotic debris with a non-absorbent cotton swab, which has been dipped in saline.
   3. Discard the swab and debris.
   4. Using a second non-absorbent cotton swab which has been moistured in saline gently rub the growing margins of the lesion.
   5. Quickly roll the swab onto a glass slide which is held over the open bottle of fixative and immediately drop the slide into the fixative.
   6. Stain with H&E / Pap stain.